Historic American Apple Cultivars: Identification and Availability

The RT-PCR identification and sequence analysis of Apple chlorotic leaf spot virus from apple cultivars in Jiaodong Peninsula, China

A set of specific primer pairs was utilized to detect Apple chlorotic leaf spot virus (ACLSV) from seven different apple cultivars in Jiaodong Peninsula via reverse transcription polymerase chain reaction (RT-PCR), and the sequence of ACLSV genome was analysed. The results indicate that: (1) High-purity total RNA could be successfully isolated using plant RNA rapid extraction kit. The ratios of...

Identification of apple cultivars on the basis of simple sequence repeat markers.

DNA markers are useful tools that play an important role in plant cultivar identification. They are usually based on polymerase chain reaction (PCR) and include simple sequence repeats (SSRs), inter-simple sequence repeats, and random amplified polymorphic DNA. However, DNA markers were not used effectively in the complete identification of plant cultivars because of the lack of known DNA finge...

SNP discovery in apple cultivars using next generation sequencing

Background Knowledge about single nucleotide polymorphism (SNP) markers is extremely important in the development of genotyping assays, allowing improvements in plant breeding through marker-assisted selection. With the emergence of next generation sequencing platforms, high-density SNP discovery in the genome of plant crops becomes more achievable. In this project, we carried out whole genome ...

Traditional versus modern apple cultivars – a comparison of juice composition

Apples are rich in components that are important for human health, such as polyphenols, organic acids and microelements, but their chemical composition varies with different apple cultivars. We aimed to find out if there is any superiority in traditionally grown apple cultivars in terms of their fruits’ bioactive components content. Juice from two traditionally grown apple cultivars, ‘Mutsu’ an...

American Self-Identification: A Strategy of Maintenance